When working with peptides, there are a lot of factors to be taken into consideration, including storing them for the short-term and long-term. That is what we will be discussing in this article to help you preserve your peptides for the longest time possible and get the best quality out of them with every use. Keep reading to find out how to store peptides.

Getting the peptides

Standard shipping methods (FEDEX, Post, DHL...) transport peptides, which are sent at room temperature. They are sent in frozen form, making them easy to store and transport. Two tubes carrying the same amount of powder might have different volumes when seen side-by-side. There should be no skepticism about the distinction here. Peptides that are very hygroscopic, such as short sequences, might appear as gels or are almost undetectable. Some companies does not specify the stability of bespoke peptides. However, this may be done upon request.

Storage for a short time

Lyophilized peptides may be stored in their original packing for many days to weeks at room temperature. Depending on personal choice and experimentation, the peptides may be kept at a temperature of 4 °C or lower. Protecting peptides from bright light is essential.

Storage for a long time

Peptides should be stored at -20 °C or -80 °C for at least four weeks before use.

Peptides may be stored in solution.

Lyophilized Peptides are more stable than those in solution. To prevent peptide damage from thaw-refreeze cycles, aliquot peptide solutions before freezing. Solvent type, pH (pH 5-7 is regarded optimum), and an amino acid sequence all have a role in solution stability. Asn, Gln, Cys, Met, Trp, and Tyr-containing peptides are less stable than those not containing Asn, Gln, Cys, Met, Trp, and Tyr. Dry (anhydride) organic solvents are excellent for preparing peptide stock solutions because they prevent early hydrolysis. Aliquots may be stable for weeks or months. Peptides in solution are usually stable for 1-2 weeks at +4°C, for 3-4 months at -20°C, and for one year at -80°C, according to worldwide consensus. Stability studies for your peptides are available online; read the "peptide stability studies" section for more details.

You should weigh the peptide as early as possible since they are generally very hygroscopic and quickly absorb water from the atmosphere. Alternatively, pre-aliquoted peptides sold online may save you time and assure correctness.

Do not handle peptides carelessly!

Some peptide companies manufacture their customers' peptides on behalf of such clients. There are no safety data sheets provided by these companies for peptides. As a result, they are immune from liability in an accident. Our customers should, however, exercise care while using peptides. Peptides that have been lyophilized have the potential to be flammable. Specific peptides may be harmful to animals, people, or the environment. Safety glasses, gloves, and masks must be worn in the laboratory. Finally, handling peptides in a clean and well-ventilated environment is critical. Keep an eye on the storage conditions of your peptides, and have fun with your studies!